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Thermo Fisher
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Promega
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Beijing Genomics Institute Shenzhen
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Oxford Nanopore
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ATCC
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Promega
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Thermo Fisher
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New England Biolabs
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Image Search Results
Journal: Frontiers in Microbiology
Article Title: Longitudinal assessment of the prevalence of Fusobacterium necrophorum , Fusobacterium var ium , and Salmonella enterica in the nasal cavity, ruminal fluid, and feces of finishing beef steers with and without liver abscesses
doi: 10.3389/fmicb.2025.1565303
Figure Lengend Snippet: Species and subspecies of Fusobacterium , genes targeted, and primer and probe sequences used in the qPCR assay.
Article Snippet: Briefly, samples were partially thawed at room temperature to transfer 100 mg of sample into ZR BashingBead™ Lysis Tubes containing 375 μL ZymoBIOMICS™ Lysis Solution, 375 μL of DNA/RNA Shield™, and 1×10 6 copies of
Techniques: Sequencing, Amplification
Journal: Nucleic Acids Research
Article Title: Single-stranded pre-methylated 5mC adapters uncover the methylation profile of plasma ultrashort Single-stranded cell-free DNA
doi: 10.1093/nar/gkae276
Figure Lengend Snippet: 5mCAdpBS-Seq protocol reduces the inclusion of DNA degradation in the ultrashort region of the final library. ( A ) Schematic of the routine BS-Seq workflow incorporates degraded cell-free DNA or genomic DNA, which enters the library, potentially masking the uscfDNA methylation signal. ( B ) 5mCAdpBS-Seq protocol in which pre-methylated adapters are attached prior to bisulfite conversion, preventing degraded DNA from entering the final library. ( C ) BRcfDNA-Seq (black), BS-Seq (pink), and 5mCAdpBS-Seq (green) protocols generate different fragment profiles for reads aligning to nuclear and ( D ) mitochondria genomes. ( E ) CpG density and ( F ) G-Quad density of 5mCAdpBS-Seq resemble the BRcfDNA-Seq profiles compared to the BS-Seq protocol. The reads that align to mitochondria contributed to the minority of the total sequence reads, averaging 0.35 ± 0.006%, 0.0135 ± 0.002% and 0.0664 ± 0.012% for BRcfDNA-Seq, BS-Seq and 5mCAdpBS-Seq, respectively. Data are presented as the mean and SEM of five paired non-cancer samples.
Article Snippet: For all reactions, 1.5 μl (1 μg) of
Techniques: Methylation, Sequencing